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MULTIPLEX ELISA ASSAYS

Cira™ Immunoassay Platform
The Cira immunoassay platform revolutionizes protein biomarker research — combining ELISA’s sensitivity and reproducibility with all the advantages of multiplexing. Only Cira™ delivers on the promise of multiplexing’s throughput and affordability without compromising the sensitivity and consistency of singleplex ELISA.

The Cira™ immunoassay platform consists of the following products and services:

Ciraplex™ assays

Highlights:

  • Simultaneous Analysis of up to 12 Protein Biomarkers per Well
  • Custom Development and Validation of Disease-Specific Panels
  • 96-well Plate-Based Arrays for High-Throughput Biomarker Screening
  • Assays in Key Therapeutic Areas: 
  • Sensitive chemiluminescent detection enables quantification of low-level proteins in a higher number of samples
  • Patent-pending circular array format avoids inconsistencies resulting from the vortex generated upon mixing, offering maximum array consistency
  • Low volume requirements (2 to 50μl for testing in duplicate) enable screening of more biomarkers with limited sample

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Aushon’s menu covers a wide breadth of critical therapeutic areas, including inflammation, oncology, cardiovascular, pulmonary, and obesity/diabetes. You can choose from various human blood biomarkers including:

  • Cytokines
  • Chemokines
  • Cytokine receptors
  • Angiogenesis factors
  • Cell adhesion molecules
  • Matrix Metalloproteinases

For preclinical work, Aushon provides the analytes from the following species:

  • Non-human primate
  • Murine
  • Rat
  • Canine
  • Porcine
  • Bovine

Chemiluminescent Catalog Kits

Chemiluminescent Custom kits

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Cirascan™ immunoassay system

Cirascan™ Imager

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Highlights:

  • Ready-to-Use
    Out-of-the-box installation. No daily start-up or shut-down procedures.
  • Sensitive
    Astronomy-grade CCD camera with matched custom lens.
  • Accurate
    Bottom-read imaging eliminates interference from meniscus. Whole-plate imaging ensures consistency across the plate.
  • Fast
    Typical image acquisition time is under 2 minutes
  • Easy-to-Use
    Fixed focus and automated calculation of optimal image time eliminate manual instrument adjustments.
    1. Insert Plate  2. Click Auto-Image  3. Analyze Image in Cirasoft
  • Maintenance-Free
    No cleaning or maintenance required.

Imager Specifications

  • Dimensions: 11” wide x 14 ¾” high x 24” deep
  • Type of CCD: Front-illuminated
  • Single Exposure Data Acquisition: 16 bit
  • Resolution: 2184 x 1472 pixels
  • Pixel Size: 6.8 x 6.8 µm
  • Single Exposure Dynamic Range: 65,000 grayscale
  • Cooling: -23⁰C
  • Electrical Configuration: 100-240 VAC/1.8 amperes/50-60 Hz

Cirasoft Analysis SoftwareTM  & Cirareports™

Highlights:

 

  • Proprietary analysis software with automated spot-finding, spot measurement, and data analysis
  • Easily create and save user-specific assay templates and plate layouts
  • Automatically generate standard curve plots, %CV for standards, standard backfits, LLOD, and LLOQ for each assay in the array
  • View mean values, %CV, standard deviation, and linear quality for each unknown sample for each analyte.
  • Standard reports present data by sample and by analyte. Samples above a user-defined %CV threshold or outside a user-defined detectable range can be flagged.



























Cirareports Study Software

Highlights:

  • Cirareports was designed to manage data from large studies involving hundreds or thousands of samples
  • Data from multiple plates of a single array panel is condensed and tabulated in one overall report, eliminating manual cutting and pasting of data.
  • Standard curves from individual plates can be overlayed in one screen.
  • Data for all samples tested for a single array panel are presented in one user-friendly report.