ORIGENE KNOCKOUT (KO) CELL LYSATES
Knockout (KO) Cell Lysates
Negative control for Western blotting application
What is KO cell lysate?
KO cell lysates are the cell homogenate in RIPA buffer made with double-knockout cell lines. The KO cell lines development was described in detail below. The protein concentration was determined with BCA assay. A vial of lysate from the parental cell line was also included as an internal control. To facilitate transportation and protein, the products are supplied as lyophilized proteins.
How was the KO cell lines developed?
OriGene teams up with EdiGene, a CRISPR innovator, who produces double-knockout genotype in a high-throughput manner. Unlike HAP1 cell line from Horizon, EdiGene KO cell lines are from the commonly used cell lines, such as HEK293T or HeLa, making the product more relevant for researchers.
What are the applications of the KO cell lysate?
The immediate utility is to serve as negative controls for western blots and ultimately to validate the specificity of antibodies.
We are expecting that novel applications such as a negative control for functional assay. Feel free to contact us if you have any ideas on how to use this material.
OriGene is the first to offer gene-specific knockout lysates for Western blotting controls.
- Genetically verified double knockout phenotypes
- Commonly used cell lines (HeLa or HEK293)
- Supplied as a kit with KO and parental protein lysates
- New lysates added monthly
- Complementary to positive controls: over-expression lysates