Cellecta’s CRISPRa and CRISPRi human genome-wide sgRNA libraries have been designed to target almost 19,000 human protein-coding genes using 5 sgRNAs designed against the promoter region based on Horbeck, et al. (eLife 2016;5:e19760 DOI: 10.7554). Our dual-guide CRISPRa and CRISPRi libraries use the same 5 effective sgRNA in dual combination on each of 5 constructs (sgRNA 1 & 2, sgRNA 1 & 3, sgRNA, sgRNA 2 & 3, etc.). Libraries are available in 200 ug plasmid and pre-packaged 2 x 10^8 TU and 1 x 10^9 TU lentiviral particles.
Human Genome-Wide CRISPRa Single-sgRNA and Dual-sgRNA Libraries
Selective activation of targeted genes when used in cells expressing dCas9-VPH (or other dCas9-activator variations compatible with spCas9 sgRNA designs)
Human Genome-Wide CRISPRi Single-sgRNA and Dual-sgRNA Libraries
Selective expression inhibition of targeted genes when used in cells expressing dCas9-KRAB (or other deactivated Cas9 repressors compatible with spCas9 sgRNA designs)
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