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Competent cells

Lucigen offers the best performance, value, and convenience in competent cells for cloning and expression. Lucigen's Chemically Competent Cells offer an unmatched value for routine cloning, and our Electrocompetent Cells provide the highest possible efficiency for difficult cloning and library construction. Lucigen also has a variety of Competent Cells for Protein Expression. No other supplier offers such performance

Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), Subcloning Grade (12 transformations per tube), or microplates as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

E. cloni® 10G and 10GF' Chemically Competent Cells

Choice of strain:

  • E. cloni 10G Competent Cells. Library construction, Routine cloning, subcloning, and plasmid isolation with or without blue/white screening.
  • E. cloni 10GF' Competent Cells. Contain the F' plasmid for infection with M13 to produce ssDNA

Choice of efficiency:

  • E. cloni 10G Chemically Competent Cells (> 1 x 109 cfu/µg pUC DNA; (> 1 x 108 in 96-well plates). Highly efficient competent cells for routine cloning applications.  Available in one transformation per tube (SOLOs) or two transformations per tube (DUOs).  Also available in 96-well plates (which can be divided into 24-well seqments) for higher throughput.
  • E. cloni 10GF' Chemically Competent Cells (> 5 x 108 cfu/µg pUC DNA).  An excellent choice for producing single-stranded DNA for sequencing, mutagenesis, etc.
  • E. cloni 10G Chemically Competent Cells, Subcloning Grade (> 1 x 106 cfu/µg pUC DNA).  The best value available anywhere for simple cloning and plasmid propagation
  • E. cloni 10G SUPREME Electrocompetent Cells > 4 x 1010 cfu/µg pUC DNA
    SUPREME Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.
  • E. cloni 10G & 10GF' ELITE Electrocompetent Cells > 2 x 1010 cfu/µg pUC DNA
    ELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.
  • E. cloni 10G CLASSIC Electrocompetent Cells > 5 x 109 cfu/µg pUC DNA
    CLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.

E. cloni 5-alpha Chemically Competent Cells

Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening.
Efficiency >1 x 108 cfu/μg DNA

 

ClearColi™ BL21(DE3) Electrocompetent Cells

  • Genetically modified LPS does not trigger endotoxic response in human cells
  • Ideal for mammalian cell immunogenicity testing, toxicity assays, and therapeutic protein drug discovery
  • Useful for membrane and lipid binding protein production
  • Protein expression similar to BL21(DE3) cells without requiring downstream endotoxin removal
  • No stimulation of hTLR4 cascade
  • Reduce false positives in cytokine assays, improve confidence in your results
  • Downstream endotoxin removal not required in most cases

Click here for more informations on ClearColi™ cells

 
 

Phage Display Competent Cells

  • Perfect for antibody phage display or peptide phage display library creation
  • The highest efficiency TG1 competent cells available: >4 x 1010 cfu/µg.
  • Your only source for electrocompetent SS320 and ER2738 cells
  • Amber suppressor and non-amber suppressor lines for maximum flexibility
  • High efficiency MC1061 F- cells for cloning or control reactions
  • Blue/White screening
  • Create larger libraries; speed discovery
  • Bulk custom dispensing available with all of our phage display cells

Choice of strain:

  • TG1 Electrocompetent Cells. An amber suppressor strain (supE) prepared as highly efficient (>4 x 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells are used for phage display and protein expression.
  • SS320 Electrocompetent Cells. A non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (>4 x 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells have the highest available transformation efficiency of any strain used for phage display.
  • MC1061 F- Electrocompetent Cells. A non-amber suppressor strain, identical to SS320 except that it lacks the F'episome. Highly efficient (>4 x 1010 cfu/µg) electrocompetent cells for general cloning or Phage Display. Note that as an F- strain, it cannot be used for re-infection by filamentous phage.  This strain not included in the sample kit.
  • ER2738 Electrocompetent Cells. An amber suppressor strain (glnV) prepared as highly efficient (>2 x 1010 cfu/µg) electrocompetent cells for phage display library screening. This strain is recommended for use with New England Biolab's Ph.D.™ Phage Display Kits.

Endura™ Competent Cells

  • Clone unstable sequences with reliable results
  • Direct replacement for Invitrogen Stbl3™ and Agilent SURE strain
  • Available as chemically competent or electrocompetent cells
  • Your choice of two packaging formats
  • High efficiency: over 1 x 108 cfu/µg (chem) or 1 x 1010 cfu/µg (electro)
  • Excellent value

BAC-Optimized Replicator™ v2.0 & 10G BAC-Optimized Electrocompetent Cells

  • Cells that enable the most challenging library construction and screening.
  • Highest available transformation efficiencies for library construction and screening.
  • Optimized for high yields of DNA.
  • Blue/White screening

Choice of strain:

  • BAC-Optimized Replicator™ v2.0 Electrocompetent Cells (> 2 x 1010 cfu/µg pKanR DNA).  These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields.  BAC-Optimized Replicator Cells are components of Lucigen’s CopyRight® Cloning Kits.
  • E. cloni 10G BAC-Optimized Electrocompetent Cells (> 1 x 107 cfu/µg 150 kb BAC DNA; > 1 x 1010 cfu/µg pUC DNA). The first competent cells developed by Lucigen specifically for large DNA cloning.  Manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts, 10G BAC-Optimized Cells are an excellent choice for BAC library construction and large construct transformation

BigEasy®-TSA™ Electrocompetent Cells

  • Cells required for transformation and propagation of pJAZZ® Vectors.
  • Highest available transformation efficiencies for library construction and screening.
  • Optimized for high yields of DNA
  • Blue/White screening

BigEasy™-TSA™ Electrocompetent Cells (>2 x 1010 cfu/µg pKanR DNA) are for use with Lucigen’s pJAZZ®-OC or OK Linear Vectors in the BigEasy v2.0 Linear Cloning System

CJ236 Electrocompetent Cells

High efficiency cells to create uracil-containing DNA for site-directed mutagenesis

  • Sole source of highly efficient Electrocompetent cells (1 x 109 cfu/µg)
  • ung- and dut- mutations to generate DNA with some uracil residues
  • Ideal genotype and efficiency for Kunkel directed mutagenesis method1.

OverExpress™ C41(DE3) and C43(DE3) Competent Cells

  • Express genes cloned into any T7 vector with these BL21(DE3) derivatives
  • Effective in expressing toxic & membrane proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals.
  • Cited in over 350 research articles
  • The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins
  • The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3).
  • pLysS are lysogens of λDE3. These strains carry a chromosomal copy of the T7 RNA Polymerase gene under the control of the lacUV5 promoter. These strains are suitable for production of protein from target genes cloned into T7-driven expression vectors.

E. cloni® EXPRESS BL21(DE3) Competent Cells

  • Best value for routine protein expression... available as Electrocompetent (> 5 x 109 cfu/µg) and Chemically Competent Cells (>1 x 107 cfu/µg)
  • Transformation efficiency
  • Eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment.
  • Phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.
  • pLysS Electrocompetent Cells (> 2 x 108 cfu/µg) produce T7 lysozyme to repress low-level transcription from the T7 promoter prior to IPTG induction, helping to stabilize inserts coding for deleterious products.
  • pLysE Electrocompetent Cells > 2 x 108 cfu/µg) have a higher level of repression by T7 lysozyme, offering the greatest control over expression of inserts.

Biotin XCell™ F' Competent Cells

Biotin XCell™ F’ cells are based on the MC1061 strain, but have had the birA gene integrated into the genome. The BirA protein (biotin ligase), which is induced with the addition of arabinose, will facilitate the addition of a biotin molecule to proteins bearing the appropriate signal sequence (AviTag). 

  • Efficient in vivo biotinylation of target protein. High efficiency electrocomp cells.
  • Sole source of highly efficient electrocompetent cells (1 x 1010 cfu/µg)
  • BirA gene stably integrated into genome for in vivo biotinylation of tagged proteins
  • Transformation efficiency is 1 x 1010 cfu/µg

HI-Control™ Competent Cells

The HI-Control™ strains are based on the popular E. cloni 10G (same as DH10B) and BL21(DE3) strains. They were created by adding a plasmid bearing a constitutive lacI gene, which provides tight control over promoters containing the lacO operator.

  • Tightly regulated protein expression for promoter systems using the lac operator
  • Compatible with T7-lac and other lac based promoters
  • HI-Control™ 10G Chemically Competent cells: > 1 x 109 cfu/µg
  • HI-Control™ BL21(DE3) Chemically Competent cells: > 1 x 108 cfu/µg
  • Both 10G and BL21(DE3)-based strains are noted for their ability to express high levels of protein.

For more informations on cloning products, please click here

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