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Difficult or BAC/Fosmid cloning

CopyRight® v2.0 BAC Cloning Kits (pSMART® BAC and pEZ™ BAC)

Lucigen's CopyRight® v2.0 BAC cloning kits with pSMART® BAC (Figure1) or pEZ BAC (Figure 2) vector and BAC-Optimized Replicator™ v2.0 Electrocompetent Cells result in unprecedented accuracy, reliability, and efficiency in BAC cloning and library construction.

See an example of a Random Shear BAC library generated with pSMART BAC.

  • Efficiently clone any size insert up to 200kb.
  • Create libraries from A/T- or G/C-rich genomes.
  • Clone gene clusters or operons.
  • High insert stability.
  • Inducible copy number.
  • Transcription-free cloning technology, which further increases insert stability
  • A lacZ-sacB stuffer fragment eliminates uncut vector from recombinants, resulting in zero background

CopyRight® v2.0 BAC Cloning Kit content

Pre-cut vector (pSMART BAC with a choice of BamHI or HindIII sites), CopyRight DNA Ligase, CopyRight 5X Ligation Buffer, Positive Control Insert DNA, Sequencing Primers, BAC-Optimized Replicator v2.0 Electrocompetent Cells in DUO packaging (2 transformations per tube), Positive Control Insert DNA, Recovery Medium, Arabinose Induction Solution, and complete protocols.

CopyRight pEZ BAC Cloning Kit content

Pre-cut CopyRight vector (pEZ BAC Blunt), CloneSmart DNA Ligase, CloneDirect™ 10X Ligation Buffer (includes ATP), Positive Control Insert DNA, Replicator Induction Solution, Sequencing Primers, and complete protocols. Kits with cells also include BAC-Optimized Replicator v2.0 Electrocompetent Cells (>2 × 1010 cfu/µg DNA) packaged in two transformations per tube (DUOs), Positive Control Plasmid, and Recovery Medium.

CopyRight® v2.0 Fosmid Cloning Kits (pSMART® FOS)

Lucigen’s CopyRight® v2.0 Fosmid Cloning Kit with the pSMART® FOS vector and Replicator™ FOS strain contains the most advanced fosmid cloning developed.

The vector incorporates CopyRight transcription terminators to stabilize inserts, which results in complete fosmid libraries without missing clones.

  • Efficiently clone any insert of 35-45 kb.
  • Get more recombinants from less DNA.
  • High insert stability.
  • No empty-vector background
  • Inducible copy number

CopyRight v2.0 Fosmid Cloning Kit content

Pre-cut, dephosphorylated pSMART FOS blunt vector, CloneSmart DNA Ligase, CloneDirect™ 10X Ligation Buffer (includes ATP), DNATerminator End Repair Enzyme Mix and End Repair Buffer, Replicator FOS strain (glycerol stock), 20% Maltose Solution, 1M MgSO4, SM Buffer, Arabinose Induction Solution, Sequencing Primers, and complete protocols. The 20-reaction Kit is provided as two 10-reaction Kits.

 

For more informations on cloning products, please click here

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