General cloning
CloneSmart® Cloning Kits (pSMART® Vectors)
- Convenient, pre-processed vectors eliminate the need for digestion, gel purification, and dephosphorylation.
- Transcription terminators flanking cloning site stabilize otherwise toxic inserts.
- Choice of copy number and antibiotic resistance.
- Available with a choice of competent cell transformation efficiencies.
- pSMART vectors are supplied pre-digested, with blunt, dephosphorylated ends, and are qualified to produce 99.5% recombinant clones in typical experiments.
CloneSmart Blunt Cloning Kits content
- Vector Premix
- CloneSmart DNA Ligase
- Positive Control Insert DNA
- Sequencing Primers
- Complete protocol
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Kits with cells also include a choice of E. cloni cells with Recovery Medium and Positive Transformation Control
- Electrocompetent ELITE 10G or 10GF' (> 2 x 1010 cfu/µg) or 10G SUPREME (> 4 x 1010 cfu/µg)
- Chemically Competent 10G (> 1 x 109 cfu/µg) or 10GF' (>5 x 108 cfu/µg)
Kits without cells can be used with any E. coli chemically competent or electrocompetent cells.
Cells in the DUO format contain 2 transformations per vial; the SOLO format is 1 transformation per vial.
For more informations on cloning products, please click here