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Mitochondrial related-events products

Although organelles remain grossly normal during the earlier stages of apoptosis, research has  revealed a pivotal role for mitochondria in the apoptotic cascade. In non-apoptotic, healthy cells, cellular energy produced during mitochondrial respiration, is maintained as an electrochemical gradient that constitutes a high mitochondrial transmembrane potential (ΔΨmhigh). The decreases in membrane potential have been used as a characteristic apoptotic marker. To detect depolarization a cationic dye can be used. The dye readily enters cells, accumulate as a multimeric aggregate and fluoresces red within healthy mitochondria. In apoptotic cells, the mitochondrial membrane potential collapses, and dye cannot accumulate within the mitochondria. In these cells, the dye returns to its green fluorescent monomeric form. Apoptotic cells, showing primarily green fluorescence, are thus easily differentiated from healthy cells which show red fluorescence (see below DePsipher kit, MitoPT JC1, MitoShift )

 

http://www.rndsystems.com/resources/images/6245.jpg

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