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Home > PROTEOMICS > ELISA/CLIA/COLORIMETRIC KITS > 20S Proteasome Activity Kit
20S Proteasome Activity Kit
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Code | SKT-133-96 |
---|---|
Description | 20S Proteasome Activity Kit |
Size | 1 x 96T |
General information | Proteasomes are non-lysosomal proteolytic complexes localised primarily in the cytoplasm and in the nucleus of eukaryotic cells. They are responsible for the ubiquitin-mediated degradation of short half-life proteins and peptides that are involved in essential cellular processes including cell-cycle regulation, apoptosis and transcriptional regulation, innate immunity and antigen processing, and in the removal of redundant or damaged proteins. As such protein degradation by the ubiquitin-proteasome pathway has a major regulatory function for proliferation activity and survival of both normal and malignant cells, and its dysfunction has been implicated in a wide range of other disease processes including neurodegenerative, cardiovascular and metabolic disorders. The 26S proteasome structure is composed of a 20S proteasome catalytic core complex and one or two 19S (PA700) regulatory subcomplexes. The 20S core comprises two copies of 14 subunits (7 alpha subunits and 7 beta subunits) arranged in a α7β7β7α7 cylindrical array. Proteolytic activities are determined by the β1 (caspase-like), β2 (trypsin-like) and β5 (chymotrypsin-like) subunits, access to which is guarded by the α-subunits. The 19S regulatory unit consists of six ATPase and at least ten non-ATPase subunits that are required for ubiquitinated protein binding, deubiquitination, substrate unfolding and translocation to the 20S catalytic core. Varying catalytic subunit composition (β1, β1i; β2, β2i; β5, β5i) results in a variety of possible subtypes from full constitutive proteasome (β1, β2, β5) through mixed populations to full inducible / immunoproteasome (β1i, β2i, β5i). Alternative regulatory complexes such as the PA200 and 11S proteasome activators confer different substrate specificities and activity compared to the 19S regulator. |
ALTERNATIVE NAME | Chymotrypsin-like (β5) Activity Kit |
Assay type | Complete kit |
Detection method | ELISA |
Sample type | Purified Proteins |
Target species | Species Independent |
Storage | 4ºC |
Data sheet | VIEW |
Research area | Cancer | Apoptosis | Cell Signaling | Post-translational Modifications | Neuroscience | Neurodegeneration | Cardiovascular System |
Source | StressMarq |
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© 1995-2024 temaricerca.com - Tutti i diritti riservati - P.IVA 00674091202
Via XXI Ottobre 1944, 11/2, 40055 Castenaso (BO) - tel: 051 62 40 700 - fax: 051 62 40 706