chiudiNGS
NGS Library Preparation
Bisulfite Sequencing
The EpiNext High-Sensitivity Bisulfite-Seq Kit (Illumina) can be used to simultaneously bisulfite-convert DNA (>99%) and prepare an Illumina-based library for Methylseq.
NGS library preparation kits for epigenetic research, please see links below
Post-Bisulfite DNA NGS Libraries
The EpiNext Bisulfite-Sequencing and EpiNext High Sensitivity Bisulfite-Seq Kits include reagents for bisulfite modification as well as for subsequent library preparation.
The EpiNext Post-Bisulfite DNA Library Preparation Kits include all reagents for post-bisulfite library preparation.
The EpiNext™ NGS Barcode (Index) Set-12 for constructing multiplex DNA libraries used for next-generation sequencing with the Illumina platform including GAIIx, HiSeq and MiSeq is compatible with both formats.
RNA bisulfite-based NGS Libraries
The EpiNext™ RNA Bisulfite-Seq Kit (Illumina) is a complete set of optimized reagents designed to carry out RNA bisulfite conversion, followed by a "post-bisulfite" library preparation process for Illumina platform-based bisulfite sequencing, all in one kit.
ChIP-Seq NGS Libraries
The EpiNext™ ChIP-Seq High-Sensitivity Kit (Illumina) is a complete set of reagents required for carrying out a successful ChIP-Seq starting from mammalian cells or tissues.
DNA NGS Libraries
xGen™ DNA Library Preparation Kits-IDT
Comprehensive coverage with fewer PCR duplicates
Streamline preparation of comprehensive NGS libraries from dsDNA. These kits utilize enzymatic fragmentation (EZ) or mechanical shearing (MC) methods to generate libraries suitable for PCR-free, PCR-amplified, and targeted sequencing research applications.
xGen™ cfDNA & FFPE DNA Library Preparation Kit
The xGen cfDNA & FFPE DNA Library Preparation Kit empowers you with highly complex variant identification from degraded and low-input research samples.
xGen™ ssDNA & Low-Input DNA Library Preparation Kit
The xGen ssDNA & Low-Input DNA Library Prep Kit enables preparation of libraries from degraded and damaged ssDNA and dsDNA samples in a single reaction. This kit allows users to sequence difficult-to-process samples via unique, proprietary AdaptaseTM technology.
RNA NGS LIBRARIES
xGen™ NGS RNA Library Preparation
Research on differential gene expression, novel fusion genes, or alternative splicing patterns involves decoding cellular RNA. The IDT xGen NGS RNA Library Preparation Kits provide comprehensive data from simple workflows to help you make the next discovery.
xGen™ Methylation-Sequencing DNA Library Preparation Kit
Comprehensive methylome coverage from low-input research samples
xGen Methyl-Seq Library Prep Kit utilizes Adaptase™ technology that efficiently captures bisulfite-converted ssDNA molecules into library molecules for epigenetic research studies. The resultant libraries represent uniform and comprehensive genome coverage.
xGen™ NGS Adapters & Indexing Primers
Target Capture (Enrichment)
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xGen™ Exome Hybridization Panel
xGen™ Custom Hybridization Capture Panels
Custom target enrichment panels for maximum flexibility
- Includes an NGS Functional Test Report† with specific capture metrics to ensure confidence
- Delivered typically in as few as 3 weeks
- Accel available in 5 business days
- Affordable options available for any size panel
- Create your custom panel design using xGen Panel Design Tool
- Reliable lot-to-lot results instills confidence in panel functionality*
- Production provides additional quality documentation, including ESI/MS on individual probes
†Not provided with the xGen Custom Hyb Panel—Accel option
*As shown in figure below
xGen Custom Hyb Panels have been optimized for use with the xGen Hybridization and Wash kit, xGen Universal Blockers, and xGen Library Amplification Primer Mix, which can be ordered from the xGen Hybridization Capture Core reagents page.
IDT can provide a solution that fits any size project. Whether it needs more design iteration, larger reaction sizes, or more quality documentation, we offer customized products that fit your research needs.
