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CLONING

Cloning is commonly used to amplify DNA fragments containing whole genes, but it can also be used to amplify any DNA sequence such as promoters, non-coding sequences and randomly fragmented DNA.

It is used in a wide array of biological experiments and practical applications ranging from genetic fingerprinting to large scale protein production.

We offer clones ready to use and a wide variety of cloning systems, including novel transcription-free cloning vectors, that improve cloning yields, reduce cloning bias, and improve sequencing throughput.

Choose from our genome-wide collection of the ready-to-use clones or let us make your customized clones by gene synthesis.

  • Comprehensive
  • Expression-ready
  • Transfection-grade
  • Flexible Shuttling

IDT and SGI-DNA have partnered to offer custom gene synthesis. Synthetic gene products manufactured by IDT and SGI-DNA can be ordered through the IDT website, allowing researchers to save money spent on construction and cloning, and saving significant time and effort by outsourcing the production of hard-to-clone sequences that often result in repeated failures.

All genes are constructed using the highest quality oligonucleotides from IDT. Genes arrive in a plasmid cloning vector and are ready for use in a variety of applications.

Gene sequences with added complexity (such as homopolymeric runs, critical hairpin structures or G/C content), which can interfere with assembly and/or sequencing performance may result in extra service charges. For more information about potential types of complexity, please see the Overview tab.

Ordering

Product Pricing
MiniGene 25-500 bp € 256,50 EUR
Gene 501-1500 bp € 0,51 EUR / bp
Gene 1501-3000 bp € 0,51 EUR / bp
Gene 3001-5000 bp € 0,68 EUR / bp
Gene 5001+ bp Inquire for Pricing

SGI-DNA is a subsidiary of Synthetic Genomics, Inc. (SGI), specializing in development, manufacturing, and commercialization of synthetic DNA. The IDT partnership with SGI-DNA will uniquely enable the research community by providing a complete solution from short genes to very large synthetic constructs with an unprecedented, fast delivery time.



Read the Press Release

 
Product Typically Shipped
MiniGene 25–500 bp  8 Business Days
Gene 501–1500 bp 12 Business Days
Gene 1501–3000 bp 18 Business Days
Gene 3001–5000 bp 25 Business Days
Gene 5001+ bp Inquire

Order Genes Now

gBlocks® Gene Fragments - Synthetic biology for any lab

gBlocks® Gene Fragments are double-stranded, sequence-verified genomic blocks up to 2000 bp in length that can be shipped in 4–7 working days for affordable and easy gene construction or modification. With gBlocks Gene Fragments, IDT has developed a new tool that makes synthetic biology easier and more accessible than ever for any lab.

Sequence information is always secure and confidential at IDT. Non-disclosure agreements are available through IDT's legal services upon request.

Ordering
Product Configuration

gBlocks© Gene Fragments Shipped (BD)1 Amount (ng) Pricing
125-250 bp 2-4 250 € 135,09 EUR
251-500 bp 2-4 500 € 135,09 EUR
501-750 bp 2-4 500 € 216,60 EUR
751-1000 bp 3-5 1000 € 270,75 EUR
1001-1250 bp 5-8 1000 € 342,95 EUR
1251-1500 bp 5-8 1000 € 415,15 EUR
1501-1750 bp 5-8 1000 € 469,30 EUR
1751-2000 bp 5-8 1000 € 523,45 EUR
Single Mixed Base 10-15 - € 266,00 EUR
>1 Mixed Bases (each) 10-15 200 € 133,00 EUR / Base

gBlocks Gene Fragments are available in lengths from 125–2000 bp.
gBlocks Gene Fragments are delivered dry, normalized to 1 ug.

Shipped within 4–7 business days—the time it takes to synthesize a gBlocks fragment is dependent on length and complexity

Order gBlocks® Gene Fragments Now

Lucigen has a wide variety of cloning systems, including novel transcription-free cloning vectors, that improve cloning yields, reduce cloning bias, and improve sequencing throughput. For cloning the most difficult sequences, the BigEasy® v2.0 Linear Cloning System offers unmatched stability. Most Lucigen cloning vectors are pre-cut, dephosphorylated, and ready-to-use.

Use the NEW ELUCIDATOR™ INTERACTIVE GUIDE to select the best cloning vector for your application

Lucigen offers the best performance, value, and convenience in competent cells for cloning and expression. Lucigen's Chemically Competent Cells offer an unmatched value for routine cloning, and our Electrocompetent Cells provide the highest possible efficiency for difficult cloning and library construction. Lucigen also has a variety of Competent Cells for Protein Expression. No other supplier offers such performance

Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), Subcloning Grade (12 transformations per tube), or microplates as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

Use the new ELUCIDATOR COMPETENT CELLS INTERACTIVE GUIDE to select the best Competent Cell type for your application

E. cloni® 10G and 10GF' Chemically Competent Cells

Choice of strain:

  • E. cloni 10G Competent Cells. Library construction, Routine cloning, subcloning, and plasmid isolation with or without blue/white screening.
  • E. cloni 10GF' Competent Cells. Contain the F' plasmid for infection with M13 to produce ssDNA

Choice of efficiency:

  • E. cloni 10G Chemically Competent Cells (> 1 x 109 cfu/µg pUC DNA; (> 1 x 108 in 96-well plates). Highly efficient competent cells for routine cloning applications.  Available in one transformation per tube (SOLOs) or two transformations per tube (DUOs).  Also available in 96-well plates (which can be divided into 24-well seqments) for higher throughput.
  • E. cloni 10GF' Chemically Competent Cells (> 5 x 108 cfu/µg pUC DNA).  An excellent choice for producing single-stranded DNA for sequencing, mutagenesis, etc.
  • E. cloni 10G Chemically Competent Cells, Subcloning Grade (> 1 x 106 cfu/µg pUC DNA).  The best value available anywhere for simple cloning and plasmid propagation
  • E. cloni 10G SUPREME Electrocompetent Cells > 4 x 1010 cfu/µg pUC DNA
    SUPREME Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.
  • E. cloni 10G & 10GF' ELITE Electrocompetent Cells > 2 x 1010 cfu/µg pUC DNA
    ELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.
  • E. cloni 10G CLASSIC Electrocompetent Cells > 5 x 109 cfu/µg pUC DNA
    CLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.

E. cloni 5-alpha Chemically Competent Cells

Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening.
Efficiency >1 x 108 cfu/μg DNA

 

ClearColi™ BL21(DE3) Electrocompetent Cells

  • Genetically modified LPS does not trigger endotoxic response in human cells
  • Ideal for mammalian cell immunogenicity testing, toxicity assays, and therapeutic protein drug discovery
  • Useful for membrane and lipid binding protein production
  • Protein expression similar to BL21(DE3) cells without requiring downstream endotoxin removal
  • No stimulation of hTLR4 cascade
  • Reduce false positives in cytokine assays, improve confidence in your results
  • Downstream endotoxin removal not required in most cases

Click here for more informations on ClearColi™ cells

 

Phage Display Competent Cells

  • Perfect for antibody phage display or peptide phage display library creation
  • The highest efficiency TG1 competent cells available: >4 x 1010 cfu/µg.
  • Your only source for electrocompetent SS320 and ER2738 cells
  • Amber suppressor and non-amber suppressor lines for maximum flexibility
  • High efficiency MC1061 F- cells for cloning or control reactions
  • Blue/White screening
  • Create larger libraries; speed discovery
  • Bulk custom dispensing available with all of our phage display cells

Choice of strain:

  • TG1 Electrocompetent Cells. An amber suppressor strain (supE) prepared as highly efficient (>4 x 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells are used for phage display and protein expression.
  • SS320 Electrocompetent Cells. A non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (>4 x 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells have the highest available transformation efficiency of any strain used for phage display.
  • MC1061 F- Electrocompetent Cells. A non-amber suppressor strain, identical to SS320 except that it lacks the F'episome. Highly efficient (>4 x 1010 cfu/µg) electrocompetent cells for general cloning or Phage Display. Note that as an F- strain, it cannot be used for re-infection by filamentous phage.  This strain not included in the sample kit.
  • ER2738 Electrocompetent Cells. An amber suppressor strain (glnV) prepared as highly efficient (>2 x 1010 cfu/µg) electrocompetent cells for phage display library screening. This strain is recommended for use with New England Biolab's Ph.D.™ Phage Display Kits.

Endura™ Competent Cells

  • Clone unstable sequences with reliable results
  • Direct replacement for Invitrogen Stbl3™ and Agilent SURE strain
  • Available as chemically competent or electrocompetent cells
  • Your choice of two packaging formats
  • High efficiency: over 1 x 108 cfu/µg (chem) or 1 x 1010 cfu/µg (electro)
  • Excellent value

BAC-Optimized Replicator™ v2.0 & 10G BAC-Optimized Electrocompetent Cells

  • Cells that enable the most challenging library construction and screening.
  • Highest available transformation efficiencies for library construction and screening.
  • Optimized for high yields of DNA.
  • Blue/White screening

Choice of strain:

  • BAC-Optimized Replicator™ v2.0 Electrocompetent Cells (> 2 x 1010 cfu/µg pKanR DNA).  These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields.  BAC-Optimized Replicator Cells are components of Lucigen’s CopyRight® Cloning Kits.
  • E. cloni 10G BAC-Optimized Electrocompetent Cells (> 1 x 107 cfu/µg 150 kb BAC DNA; > 1 x 1010 cfu/µg pUC DNA). The first competent cells developed by Lucigen specifically for large DNA cloning.  Manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts, 10G BAC-Optimized Cells are an excellent choice for BAC library construction and large construct transformation

BigEasy®-TSA™ Electrocompetent Cells

  • Cells required for transformation and propagation of pJAZZ® Vectors.
  • Highest available transformation efficiencies for library construction and screening.
  • Optimized for high yields of DNA
  • Blue/White screening

BigEasy™-TSA™ Electrocompetent Cells (>2 x 1010 cfu/µg pKanR DNA) are for use with Lucigen’s pJAZZ®-OC or OK Linear Vectors in the BigEasy v2.0 Linear Cloning System

CJ236 Electrocompetent Cells

High efficiency cells to create uracil-containing DNA for site-directed mutagenesis

  • Sole source of highly efficient Electrocompetent cells (1 x 109 cfu/µg)
  • ung- and dut- mutations to generate DNA with some uracil residues
  • Ideal genotype and efficiency for Kunkel directed mutagenesis method1.

OverExpress™ C41(DE3) and C43(DE3) Competent Cells

  • Express genes cloned into any T7 vector with these BL21(DE3) derivatives
  • Effective in expressing toxic & membrane proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals.
  • Cited in over 350 research articles
  • The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins
  • The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3).
  • pLysS are lysogens of λDE3. These strains carry a chromosomal copy of the T7 RNA Polymerase gene under the control of the lacUV5 promoter. These strains are suitable for production of protein from target genes cloned into T7-driven expression vectors.

E. cloni® EXPRESS BL21(DE3) Competent Cells

  • Best value for routine protein expression... available as Electrocompetent (> 5 x 109 cfu/µg) and Chemically Competent Cells (>1 x 107 cfu/µg)
  • Transformation efficiency
  • Eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment.
  • Phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.
  • pLysS Electrocompetent Cells (> 2 x 108 cfu/µg) produce T7 lysozyme to repress low-level transcription from the T7 promoter prior to IPTG induction, helping to stabilize inserts coding for deleterious products.
  • pLysE Electrocompetent Cells > 2 x 108 cfu/µg) have a higher level of repression by T7 lysozyme, offering the greatest control over expression of inserts.

Biotin XCell™ F' Competent Cells

Biotin XCell™ F’ cells are based on the MC1061 strain, but have had the birA gene integrated into the genome. The BirA protein (biotin ligase), which is induced with the addition of arabinose, will facilitate the addition of a biotin molecule to proteins bearing the appropriate signal sequence (AviTag). 

  • Efficient in vivo biotinylation of target protein. High efficiency electrocomp cells.
  • Sole source of highly efficient electrocompetent cells (1 x 1010 cfu/µg)
  • BirA gene stably integrated into genome for in vivo biotinylation of tagged proteins
  • Transformation efficiency is 1 x 1010 cfu/µg

HI-Control™ Competent Cells

The HI-Control™ strains are based on the popular E. cloni 10G (same as DH10B) and BL21(DE3) strains. They were created by adding a plasmid bearing a constitutive lacI gene, which provides tight control over promoters containing the lacO operator.

  • Tightly regulated protein expression for promoter systems using the lac operator
  • Compatible with T7-lac and other lac based promoters
  • HI-Control™ 10G Chemically Competent cells: > 1 x 109 cfu/µg
  • HI-Control™ BL21(DE3) Chemically Competent cells: > 1 x 108 cfu/µg
  • Both 10G and BL21(DE3)-based strains are noted for their ability to express high levels of protein.

Lentiviral system for efficient DNA delivery (Lenti shRNA & LentiORF)

OriGene is licensed to provide lentiviral vectors and related products.

  • Third generation Lentiviral system
  • Ideal for delivering DNA into hard-to-transfect and animal models
  • Replication-incompetent system for safety>
  • Improved biosafety features

Both shRNA and ORF lentiviral particles are offered!

  • Ready-to-Use: Just add to cells and get results
  • Coverage: Genome-wide, Human, Mouse and Rat
  • Broad spectrum: Transduce dividing, non-dividing cells and in vivo
  • Biosafety: Replication deficient 3rd generation lenti particles