The semplicity of transfection
What is transfection?
Traditionally, transfection has been the introduction of foreign DNA by non-viral means into cultured eukaryotic cells. Currently, the term transfection applies to the introduction of any naked nucleic acid molecule, not just DNA, into cultured eukaryotic cells.
optimizing transfection Performance General suggestions for all reagents, cells and nucleic acids
- ratio of transfection reagent to nucleic acid For each cell type, simultaneously transfect different amounts of nucleic acid using various amounts of transfection reagent.
- transfection complex formation time Test different transfection complex formation times (reagent + nucleic acid incubation time) for each reagent and cell type being transfected.
- cell density (confluency) at time of transfection Transfect each cell type at various densities ranging from 50% to 90% and monitor transfection performance.
- Post-transfection incubation time (time after addition of transfection complexes to cells) Harvest cells at different times post-transfection to determine the optimal assay time based on the experimental goals.
The TransIT® Transfection Reagents are ideal for delivering all types of nucleic acids including: DNA, siRNA, miRNA, mRNA, viral RNA and oligonucleotides to eukaryotic cells. Each reagent provides high efficiency transfection while exhibiting low cellular toxicity, enabling the acquisition of physiologically relevant data and preservation of the transfected cell population.
DNA and siRNA/miRNA
TransIT-X2™ Dynamic Delivery System
TransIT-PRO® Transfection Kit
CHOgro® Expression System
Breast Cancer Cell Transfection
Viral RNA, mRNA
DNA and RNA
Mirus Bio has developed the Ingenio® Electroporation Kits and Solution which extend the breadth of our nucleic acid delivery products. The Ingenio products facilitate efficient and reliable delivery of nucleic acids to difficult to transfect cells and are compatible with all electroporation instruments.
KITS AND SOLUTION
Ingenio® Electroporation Kits
Ingenio® Electroporation Solution
CUVETTES & DROPPERS
Ingenio® Cell Droppers