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TRANSFECTION

The semplicity of transfection

What is transfection?

Traditionally, transfection has been the introduction of foreign DNA by non-viral means into cultured eukaryotic cells. Currently, the term transfection applies to the introduction of any naked nucleic acid molecule, not just DNA, into cultured eukaryotic cells.

optimizing transfection Performance ­ General suggestions for all reagents, cells and nucleic acids

  1. ratio of transfection reagent to nucleic acid For each cell type, simultaneously transfect different amounts of nucleic acid using various amounts of transfection reagent.
  2. transfection complex formation time Test different transfection complex formation times (reagent + nucleic acid incubation time) for each reagent and cell type being transfected.
  3. cell density (confluency) at time of transfection Transfect each cell type at various densities ranging from 50% to 90% and monitor transfection performance.
  4. Post-transfection incubation time (time after addition of transfection complexes to cells) Harvest cells at different times post-transfection to determine the optimal assay time based on the experimental goals.

transfection

The TransIT® Transfection Reagents are ideal for delivering all types of nucleic acids including: DNA, siRNA, miRNA, mRNA, viral RNA and oligonucleotides to eukaryotic cells. Each reagent provides high efficiency transfection while exhibiting low cellular toxicity, enabling the acquisition of physiologically relevant data and preservation of the transfected cell population.


DNA and siRNA/miRNA

Broad Spectrum
TransIT-X2™ Dynamic Delivery System


DNA

Broad Spectrum
TransIT®-2020 Reagent TransIT®-LT1 Reagent TransIT®-Express Reagent

Protein Production
TransIT-PRO® Transfection Kit
CHOgro® Expression System

 

Breast Cancer Cell Transfection
TransIT®-BrCa Reagent

Other Cell Line Specific
TransIT®-293 Reagent
TransIT®-CHO Kit
TransIT-HeLaMONSTER® Kit
TransIT®-Jurkat Reagent
TransIT®-Keratinocyte Reagent

Insect Cell Transfection
TransIT® Insect Reagent
flashBACTM Expression System
pOET Trasfer Plasmid
 

RNA

Viral RNA, mRNA
TransIT®-mRNA Kit

siRNA/miRNA
TransIT-TKO® Reagent
TransIT-siQUEST® Reagent


OLIGO

DNA and RNA
TransIT®-Oligo Reagent


ACCESSORIES

Reporter Assay
Beta-Gal Staining Kit

Plasmid Cleanup
MiraCLEAN® Endotoxin Removal Kit


Labeled Controls

Label IT® Plasmid Delivery Controls
Cy™3
Fluorescein

Label IT® RNAi Delivery Controls
Cy™3
Fluorescein

Mirus Bio has developed the Ingenio® Electroporation Kits and Solution which extend the breadth of our nucleic acid delivery products. The Ingenio products facilitate efficient and reliable delivery of nucleic acids to difficult to transfect cells and are compatible with all electroporation instruments.


KITS AND SOLUTION

Complete Kits
Ingenio® Electroporation Kits

Electroporation Solution
Ingenio® Electroporation Solution


CUVETTES & DROPPERS

Cuvettes
Ingenio® Cuvettes

Cell Droppers
Ingenio® Cell Droppers


ACCESSORIES

Reporter Assay
Beta-Gal Staining Kit

Plasmid Cleanup
MiraCLEAN® Endotoxin Removal Kit


Labeled Controls

Label IT® Plasmid Delivery Controls
Cy™3
Fluorescein

Label IT® RNAi Delivery Controls
Cy™3
Fluorescein