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FLOW CYTOMETRY

Flow cytometry is a powerful technology for investigating many aspects of cell biology and for isolating cells of interest. Flow cytometry utilizes highly focused, extremely bright beams of light (usually from lasers) to directly reveal aspects of cells - e.g size and granularity - by the way light is scattered or indirectly by attaching fluorescent probes to cell components e.g DNA binding dyes or labeled antibodies. The power of flow cytometry derives from the fact that it quantitatively analyzes individual cells, thus permitting the identification of subpopulations within a sample. The power of single cell analysis is compounded by the ability to measure multiple parameters simultaneously on each individual cell, to do this very fast (in excess of 20,000 cells/second), and to isolate/purify/sort desired subpopulations (up to 4 simultaneously).

Current applications in flow cytometry extend far beyond traditional lymphocyte immunophenotyping. Flow cytometry is used for such varied applications as cell cycle analysis, telomere length determination, microparticle analysis, and in phagocytosis and many other studies. We offer standards to support these types of assays, for the standardized instrument set-up and comprehensive quality assurance essential for achieving accurate and consistent results within a study, and generating comparable data between instruments and laboratories



Flow cytometry Technical Guide
Flow Tutorial





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