Useful to detect active Caspase. This in vitro assay employs a fluorescent inhibitor probe to label active caspase enzyme in living cells or tissue samples. The fluorescent signal is analyzed using fluorescence microscopy, a fluorescent plate reader, or by flow cytometry.
- FLICA (Fluorescent Labeled Inhibitor of Caspases) probes are comprised of an inhibitor peptide sequence that binds to active caspase enzymes, a fluoromethyl ketone (FMK) moiety that results in an irreversible binding event with the enzyme, and a fluorescent tag (either carboxyfluorescein or sulforhodamine B) reporter. Detection of nuclear morphology and necrosis is also possible with the additional kit components, Hoechst 33342 and Propidium Iodide, respectively.