chiudiAffinity Ligand Coated Microspheres
When circumstances call for the rapid development of microsphere reagents, particles coated with affinity binding proteins offer a ready solution. Affinity binding protocols are straightforward, typically involving a simple incubation of the spheres with the appropriate ligand. Binding proteins also provide an opportunity to optimally orient the ligand, as with the end-point immobilization of biotinylated oligonucleotides to streptavidin-coated microspheres, or the Fc-specific immobilization of antibodies via Protein A or G. For more informations See TechNote 101, ProActive® Microspheres
Our streptavidin and SuperAvidin™ coated microspheres offer simple and extremely stable binding of biotinylated molecules, such as antibodies, peptides, and oligonucleotides for use in diagnostics, screening, and affinity purifications.
Our biotin-coated microspheres have been fully characterized in terms of their ability to bind free avidin, and therefore will require minimal optimization when determining the correct concentration of ligand to be bound.
Protein A and Protein G are proteins that readily bind the Fc regions of many types of antibodies. For this reason, they are used extensively for affinity purification of antibodies from serum or cell culture supernatant, and for capture of immunoprecipitated protein complexes. When Protein A and Protein G microspheres are used as reagents in tests and assays, the immobilized antibodies may be crosslinked to the Protein A- or Protein G-coated support for long-term stability.
Secondary antibody coated microspheres, using proteins such as goat anti-mouse IgG, are well-suited for many applications where streptavidin or Protein A coated microspheres are less than optimal. These have been used in immunoassay and cell separation applications, primarily when capturing antibodies for which other generic binding proteins are inappropriate.
* The final character of the catalog code denotes color: B = Blue, R = Red, O = Orange, K = Black, V = Purple, G = Green, F = Fluorescent, N = None
