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Accessory Reagents

Bangs Bead Coupling Buffers are ready-to-use buffers that are available in a variety of pH levels (4.5 to 9.0). They can be used as coupling or wash buffers for plain, dyed, or functionalized polymer microspheres.

Microspheres are routinely coated with ligands such as antibodies, oligonucleotides, and peptides, for use in diagnostics and bioseparations. Carboxyl- and amine-modified microspheres require the use of a chemical linker for activation and covalent immobilization of ligand. DEPC-Carbodiimide (EDAC) and glutaraldehyde are two of the most common crosslinkers used in bead coating protocols.

EDAC is a zero-length crosslinker that is routinely used for the covalent binding of amine-containing ligands to carboxylated microspheres, while Glutaraldehyde is a homobifunctional linker that is suitable for binding amine-containing ligands to amine-modified beads.

The Bangs Bead Solution is a ready-to-use aqueous suspending solution for the dilution and/or storage of your uncoated plain, dyed, or functionalized polymer microspheres (both carboxylated or aminated). An antimicrobial agent deters microbial contamination, and stabilizers promote suspension dispersity, peace of mind, and harmonious accord in the laboratory.

Surfactants are commonly utilized with microspheres composed of hydrophobic polymer matrices such as polystyrene to facilitate bead wetting and to deter or treat aggregation. Common concentrations are ˜0.1% for uncoated polymer beads and ˜0.01% in the storage buffer of coated polymer, magnetic, and silica beads.

Triton® X-100 and Tween® 20 are nonionic surfactants used in the storage buffers of coated bead suspensions, while Sodium Dodecyl Sulfate (SDS) is an anionic surfactant that decreases hydrophobicity and participates in charge stabilization of the suspension.

Our PolyLink Protein Coupling Kit is ideal for investigators who are new to microsphere coating, or simply desire the convenience of a kit. The kit is intended for use with carboxyl-functionalized microspheres of 1µm or larger. It contains sufficient EDAC, coupling buffer, and wash/storage buffer for 50 reactions using ˜200-500µg protein and 12.5mg COOH-functionalized microspheres per reaction.

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Product Description Applications Size Product #
Bangs Bead Coupling Buffer, pH 4.5 Coupling and Storage Buffers 250 ml BUFF1
Bangs Bead Coupling Buffer, pH 4.5 Coupling and Storage Buffers 500 ml BUFF1
Bangs Bead Coupling Buffer, pH 4.5 Coupling and Storage Buffers 1000 ml BUFF1
Bangs Bead Coupling Buffer, pH 4.5 Coupling and Storage Buffers 2000 ml BUFF1
Bangs Bead Coupling Buffer, pH 6.0 Coupling and Storage Buffers 250 ml BUFF2
Bangs Bead Coupling Buffer, pH 6.0 Coupling and Storage Buffers 500 ml BUFF2
Bangs Bead Coupling Buffer, pH 6.0 Coupling and Storage Buffers 1000 ml BUFF2
Bangs Bead Coupling Buffer, pH 6.0 Coupling and Storage Buffers 2000 ml BUFF2
Bangs Bead Coupling Buffer, pH 7.4 Coupling and Storage Buffers 250 ml BUFF3
Bangs Bead Coupling Buffer, pH 7.4 Coupling and Storage Buffers 500 ml BUFF3
Bangs Bead Coupling Buffer, pH 7.4 Coupling and Storage Buffers 1000 ml BUFF3
Bangs Bead Coupling Buffer, pH 7.4 Coupling and Storage Buffers 2000 ml BUFF3
Bangs Bead Coupling Buffer, pH 9.0 Coupling and Storage Buffers 250 ml BUFF4
Bangs Bead Coupling Buffer, pH 9.0 Coupling and Storage Buffers 500 ml BUFF4
Bangs Bead Coupling Buffer, pH 9.0 Coupling and Storage Buffers 1000 ml BUFF4
Bangs Bead Coupling Buffer, pH 9.0 Coupling and Storage Buffers 2000 ml BUFF4
Bangs Bead Solution Solution 500 ml SOLN1
Bangs Bead Solution Solution 1000 ml SOLN1
Bangs Bead Solution Solution 2000 ml SOLN1
Bangs Bead Storage Buffer, pH 7.4 Coupling and Storage Buffers 250 ml BUFF5
Bangs Bead Storage Buffer, pH 7.4 Coupling and Storage Buffers 500 ml BUFF5
Bangs Bead Storage Buffer, pH 7.4 Coupling and Storage Buffers 1000 ml BUFF5
Bangs Bead Storage Buffer, pH 7.4 Coupling and Storage Buffers 2000 ml BUFF5
Bangs Bead Storage Buffer, pH 8.5 Coupling and Storage Buffers 250 ml BUFF6
Bangs Bead Storage Buffer, pH 8.5 Coupling and Storage Buffers 500 ml BUFF6
Bangs Bead Storage Buffer, pH 8.5 Coupling and Storage Buffers 1000 ml BUFF6
Bangs Bead Storage Buffer, pH 8.5 Coupling and Storage Buffers 2000 ml BUFF6
DEPC-Carbodiimide (EDAC) Coupling Reagents 1g AA010
DEPC-Carbodiimide (EDAC) Coupling Reagents 5g AA010
Glutaraldehyde, EM Grade, 25% Coupling Reagents 100 ml AA012
Sodium Dodecyl Sulfate Anionic Surfactant Surfactants 10g AA018
Triton® X-100 Nonionic Surfactant Surfactants 10g AA014
Tween® 20 Nonionic Surfactant Surfactants 10g AA016
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