chiudiIn vitro Transfection
A tool designed to help you determine the best delivery solution for any nucleic acid into any cell type including hard-to-transfect cell lines and primary cells.
Reagent Agent® recommendations are based on extensive in-house transfection and electroporation data, customer feedback, and citations that are all continually updated.
Click at the link above and find your best transfection solution!
Use TransIT® Transfection Reagents
The Mirus TransIT® Transfection Reagents are ideal for delivering all types of nucleic acids including: DNA, siRNA, miRNA, mRNA, viral RNA and oligonucleotides to eukaryotic cells. Each reagent provides high efficiency transfection while exhibiting low cellular toxicity, enabling the acquisition of physiologically relevant data and preservation of the transfected cell population.
Understanding the impact of transfection mediated toxicity is important for every researcher. Observed toxicity is largely dependent on the transfection reagent and cell type. Choosing a transfection reagent that balances high efficiency nucleic acid delivery and low cellular toxicity is imperative to achieving reliable experimental results. Providing researchers gene delivery expertise since 1995, Mirus TransIT® transfection reagents give researchers:
- High efficiency, low toxicity transfections
- Minimal off-target effects
- Reduction in experimental bias
Mirus Broad Spectrum Transfection Reagents, TransIT®-LT1 and TransIT®-2020 Outperform Lipofectamine® 2000. HeLa cells were transfected with a GFP encoding plasmid DNA using either TransIT®-LT1, TransIT®-2020 or Lipofectamine® 2000 at reagent-to-DNA ratios of 3:1 for all reagents. Phase contrast and GFP images were taken in the same field of view at 8 and 24 hours post-transfection. Cells transfected with TransIT®-LT1, TransIT®-2020 remain healthy after 24 hours while maintaining high transfection efficiency, whereas cells transfected with Lipofectamine® 2000 display significant cytotoxicity after 24 hours.
TransIT Broad Spectrum Reagents Balance High Efficiency Delivery with Low Toxicity. HeLa cells were transfected with luciferase encoding plasmid DNA using either TransIT®-LT1, TransIT®-2020 or Lipofectamine® 2000 for 24 hours. Transfection was measured by luciferase activity using a conventional assay. Cytotoxicity was assessed by quantifying the LDH released from the cytosol of damaged cells compared to cells alone. Higher transfection efficiency and lower cytotoxicity was observed in cells transfected with TransIT®-LT1 and TransIT®-2020 at optimal ratios than cells transfected with Lipofectamine® 2000.
You can find at the following links the Mirus products divided for applications
